CRL-1657 R 1610 倉(cāng)鼠肺細(xì)胞，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件

CRL-1657 R 1610 倉(cāng)鼠肺細(xì)胞 的詳細(xì)介紹

CRL-1657

ATCC® Number:

Shipped:  frozen CRL-1657™

Designations:  R 1610

Depositors:   M Rosenberg

Biosafety Level: 1

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Cricetulus griseus （hamster, Chinese）

Morphology: fibroblast

Source: Organ: lung

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Applications: transfection host

Gender:  male

Comments: This line is a clonal derivative of V79 cells （see also V79-4, ATCC CCL-93） that had been treated with ethylmethanesulfonate.

The cells are deficient in galactokinase and are resistant to 2-deoxygalactose and 8-azaguanine.

This is a Chinese hamster somatic cell line.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liqid nitrogen vapor phase

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC CCL-93

References: 10111: Schumperli D, et al. Efficient expression of Escherichia coli galactokinase gene in mammalian cells. Proc. Natl. Acad. Sci. USA 79: 257-261, 1982. PubMed: 6281760

25665: Thirion JP, et al. Galactokinase mutants of Chinese hamster somatic cells resistant to 2-deoxygalactose. Genetics 83: 137-147, 1976. PubMed: 1269916

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