HB-198 F4/80 雜交瘤細(xì)胞， ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件

HB-198 F4/80 雜交瘤細(xì)胞

ATCC® Number: HB-198™ Price: $399.00

Designations: F4/80

Depositors: S Gordon

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus （B cell）; Mus musculus （myeloma） （rat （B cell）; mouse （myeloma））

Morphology: lymphoblast

HB-198

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against mouse macrophage

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Tumorigenic: No

Comments: Animals were immunized with thioglycollate elicited mouse peritoneal macrophages.

Spleen cells were fused with NS1 myeloma cells.

The antibody reacts specifically with mouse macrophages in many tissue sites （liver, spleen, bone marrow, brain）, and precipitates a 160000 dalton glycoprotein （function unknown）.

At the request of Dr. Gordon, the line was deposited with the ATCC in 1988 by Dr. Ralph Steinman of the Rockefeller University.

Tested and found negative for ectromelia virus （mousepox）.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1635: Hume DA, et al. The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs. J. Exp. Med. 158: 1522-1536, 1983. PubMed: 6355361

1636: Austyn JM, Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol. 11: 805-815, 1981. PubMed: 7308288

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